Cambisol Mycobiome in a Long-Term Field Experiment with Korean Pine as a Sole Edificator: A Case Study

A culture-independent mycobiome survey in Haplic Cambisol under Korean pine a long-term field experiment the Russian Far East was conducted using sequence analysis of ITS region amplified with ITS3/ITS4 primers metagenomic DNA as matrix. Overall 758 fungal OTUs were identified, representing 15 phyla, 47 classes, 104 orders, 183 families, and 258 genera. More represented Ascomycota phylum (513) than Basidiomycota (113), both phyla together comprising 95% relative abundance. The Leotiomycetes class ultimately prevailing; apparently contributing significantly to organic matter decomposition microbial biomass soil, shown by PCA. Only two dominant (Pseudogymnoascus sp. Hyaloscyphaceae, Ascomycota) common studied samples. presented high diversity soil monospecies artificial forest, where had been sole edificator for forty years, allows concluding that plant chemistry is main factor shaping such an environment. obtained data provide reference further studies mycobiota, especially its aesthetic, well nut-producing, potential. results can be helpful targeted creating beneficial pines afforestation remediation contexts.